Rani, A and Singh, K and Ahuja, Paramvir Singh and Kumar, Sanjay (2012) Molecular regulation of catechins biosynthesis in tea [Camellia sinensis (L.) O. Kuntze. Gene, 495. pp. 205-210.

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Abstract

Catechins are bioprospecting molecules present in tea and any effort towards metabolic engineering of this important moiety would require knowledge on gene regulation. These are synthesized through the activities of phenylpropanoid and flavonoid pathways. Expression regulation of various genes of these pathways namely phenylalanine ammonia-lyase (CsPAL), cinnamate 4-hydroxylase (CsC4H), p-coumarate:CoA ligase (Cs4CL), flavanone 3-hydroxylase (CsF3H), dihydroflavonol 4-reductase (CsDFR) and anthocyanidin reductase (CsANR) was accomplished previously. In depth analyses of the remaining genes namely, chalcone synthase (CsCHS), chalcone isomerase (CsCHI), flavonoid 3′5′-hydroxylase (CsF3′5′H) and anthocyanidin synthase (CsANS) were lacking. The objective of the work was to clone and analyze these genes so as to generate a comprehensive knowledge on the critical genes of catechins biosynthesis pathway. Gene expression analysis was carried out in response to leaf age and external cues (drought stress, abscisic acid, gibberellic acid treatments and wounding). A holistic analysis suggested that CsCHI, CsF3H, CsDFR, CsANS and CsANR were amongst the critical regulatory genes in regulating catechins content.

Item Type:	Article
Uncontrolled Keywords:	Anthocyanidin synthase Chalcone isomerase Flavonoid Flavonoid 3′5′-hydroxylase Gene expression
Subjects:	Plant sciences Plant Biotechnology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	03 Jan 2013 10:33
Last Modified:	03 Jan 2013 10:33
URI:	http://ihbt.csircentral.net/id/eprint/1222

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