Kumar, A and Dutt, Som and Bagler, Ganesh and Ahuja, Paramvir Singh and Kumar, Sanjay (2012) Engineering a thermo-stable superoxide dismutase functional at sub-zero to .50uC, which also tolerates autoclaving. Scientific Reports (Nature Publishing Group), 2. p. 387.

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Superoxide dismutase (SOD) is a critical enzyme associated with controlling oxygen toxicity arising out of oxidative stress in any living system. A hyper-thermostable SOD isolated from a polyextremophile higher plant Potentilla atrosanguinea Lodd. var. argyrophylla (Wall. ex Lehm.) was engineered by mutation of a single amino acid that enhanced the thermostability of the enzyme to twofold. The engineered enzyme was functional from sub-zero temperature to .506C, tolerated autoclaving (heating at 1216C, at a pressure of 1.1 kg per square cm for 20 min) and was resistant to proteolysis. The present work is the first example to enhance the thermostability of a hyper-thermostable protein and has potential to application to other proteins for enhancing thermostability.

Item Type: Article
Uncontrolled Keywords: Engineering thermo-stable superoxide dismutase functional .50uC, tolerates autoclaving
Subjects: Plant sciences
Natural Product Chemistry
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 03 Jan 2013 10:30
Last Modified: 03 Jan 2013 10:30
URI: http://ihbt.csircentral.net/id/eprint/1216

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