Sharma , H and Kumar, R and Sharma , V and Kumar , V and Bhardwaj, P and Ahuja , Paramvir Singh and Sharma, R K (2011) IDENTIFICATION AND CROSS-SPECIES TRANSFERABILITY OF 112 NOVEL UNIGENE-DERIVED MICROSATELLITE MARKERS IN TEA ( CAMELLIA SINENSIS ). American Journal of Botany, 98 (6). E-133-E-138.

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Premise of the study: Tea Unigene-derived MicroSatellite (TUGMS) markers were identifi ed from the publicly available EST data in Camellia sinensis for characterization and future genome mapping studies in tea. • Methods and Results: One hundred twelve novel TUGMS markers were identifi ed from 4356 unigenes derived by clustering of 12 788 random ESTs in C. sinensis . Amplifi cation-based validation of the TUGMS loci proved them to be highly polymorphic [an average (av.) of 5.24 alleles], heterozygous ( H E , av. 0.746; H O , av. 0.566) and informative (PIC, av. 0.392). TUGMS loci were 100% transferable in cultivated C. assamica and C. assamica subsp. lasiocalyx and highly cross-transferrable to the related species C. japonica , C. rosifl ora, and C. sasanqua . • Conclusions: These 112 novel highly polymorphic TUGMS markers with proven cross-species transferability will facilitate the future genetic diversity and genome mapping studies in tea.

Item Type: Article
Uncontrolled Keywords: Camellia sinensis ; cross-transferability; Tea Unigene-derived MicroSatellite (TUGMS).
Subjects: Plant sciences
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 20 Jun 2012 06:03
Last Modified: 20 Jun 2012 06:03

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