Pathania, V and Gupta , A P and Singh , Bikram (2006) Improved HPTLC Method for Determination of Curcuminoids from Curcuma longa. Journal of Liquid Chromatography & Related Technologies, 29. pp. 875-885. ISSN 1082-6076

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A rapid, improved, simple, and precise high performance thin layer chromatography method has been developed for the quantification of curcuminoids in Curcuma longa. Separation of curcuminoids was achieved by employing a mobile phase consisting of chloroform-methanol (98 : 2 v/v) on precoated HPTLC LiChrosphere aluminium plates Si 60F254. Densitometry analysis was carried out at 366 nm in adsorption reflection mode. The method was validated for precision and recovery. Use of LiChrosphere HPTLC plates gave better resolution, reproducibility, good selectivity and compact band as compared to other plates. Plant material collected from midhills showed higher contents of curcuminoids.

Item Type: Article
Uncontrolled Keywords: Curcuma longa, HPTLC, Curcumin, Demethoxycurcumin, Bis-demethoxycurumin
Subjects: Natural Product Chemistry
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 27 Dec 2011 10:03
Last Modified: 27 Dec 2011 10:03

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