Guleria, Praveen and Yadav , S K (2011) Identification of miR414 and Expression Analysis of Conserved miRNAs from Stevia rebaudiana. Genomics Proteomics Bioinformatics, 9 (6). pp. 211-217.

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MicroRNAs (miRNAs) usually contain 19-24 nucleotides and have been identified as important eukaryotic gene regulators. Applications of various computational approaches have simplified the task by predicting miRNAs from available sequence data sources. In this study, we identified a conserved miR414 from a computational analysis of EST sequence data available from Stevia rebaudiana. In addition, we also identified six conserved miRNAs namely miR169, miR319, miR414, miR164, miR167 and miR398 using stem-loop RT-PCR analysis. Hence, miR414 was commonly identified using both methods. The expression analysis of these miRNAs documented their roles in growth and development of Stevia. Furthermore, the detected miRNAs were found to target genes involved in plant growth, development, metabolism and signal transduction. This is the first study reporting these conserved miRNAs and their expression in Stevia.

Item Type: Article
Uncontrolled Keywords: Stevia, miRNA, computational prediction, expression analysis, target prediction, stem-loop reverse transcription
Subjects: Plant sciences
Plant Biotechnology
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 27 Apr 2012 05:27
Last Modified: 27 Apr 2012 05:27

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